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KD-Validated βIII-Tubulin Mouse mAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IHC-P, IF/ICC, ELISA |
| Host Species: | Mouse |
| Isotype: | IgG2b,kappa |
| Clonality: | Monoclonal antibody |
| Gene Name: | tubulin beta 3 class III |
| Gene Symbol: | TUBB3 |
| Synonyms: | CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A; [KD Validated] βIII-Tubulin |
| Gene ID: | 10381 |
| UniProt ID: | Q13509 |
| Clone ID: | 9Z3T0 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human TUBB3 (Q13509). |
| Dilution: | WB 1:500-1:2000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.99 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-TUBB3 monoclonal antibody |
Background
This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6.
Images
 | Western blot analysis of lysates from wild type(WT) and βIII-Tubulin knockdown (KD) HeLa cells, using [KD Validated] βIII-Tubulin Mouse mAb (A18132) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using [KD Validated] βIII-Tubulin Mouse mAb (A18132) at a dilution of 1:4500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KD Validated] βIII-Tubulin Mouse mAb (A18132) at a dilution of 1:4500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using [KD Validated] βIII-Tubulin Mouse mAb (A18132) at a dilution of 1:4500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human brain tissue using [KD Validated] βIII-Tubulin Mouse mAb (A18132) at a dilution of 1:4500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining. |
 | Immunofluorescence analysis of paraffin-embedded mouse brain using [KO Validated] βIII-Tubulin Mouse mAb (A18132) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining. |
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